Nature Structural & Molecular Biology Paper Online!
Our paper Mechanistic insight into substrate processing and allosteric inhibition of human p97 is online. In this study, we determined a series of high-resolution cryo-EM structures of p97 in substrate-engaged states and inhibitor(NMS-873)-bound states. They reveal critical conformaitonal changes of the intersubunit signaling (ISS) motifs, which contribute to the sprial staircase conformation of the translocating p97 and the allosteric inhibition of NMS-873. This is the first time human p97 is observed in asymmetric working states. We are very pround of our structures and findings!